The College of Life Sciences

Bridgewater, Laura

Microbiology & Molecular Biology

Email: 3odxudbeulgjhzdwhuCe|x1hgx  (Email Form)

Website: http://bridgewaterlab.byu.edu

3141  LSB
Provo, UT 84602
(801) 422-2434

Associate Professor

Curriculum Vitae

 Teaching Interests
Molecular Biology, Gene Regulation, Genes and Cancer
 Research Interests
One major focus of research in the Bridgewater Lab is the novel nuclear variant of BMP2, which we discovered in 2010 and named nBMP2. BMP2 has previously been recognized as a secreted growth factor that participates in bone and cartilage formation, limb formation, patterning, heart development, and apoptosis. We have discovered another form of BMP2 that is translated from the same mRNA transcript but from an alternative downstream start codon, which eliminates the signal peptide and thus prevents trafficking through the endoplasmic reticulum (ER) and Golgi. Instead, translation occurs in the cytoplasm and a nuclear localization signal directs translocation to the nucleus. We have generated a “knock-in” mouse line in which nuclear translocation of nBMP2 is blocked while secreted BMP2 is produced normally. Preliminary studies on this mouse line suggest that nBMP2 affects skeletal muscle contractions, learning and memory, cell cycle progression, and spleen development. Each of these areas has thus become a target of ongoing research in the lab.

A second area of emphasis is the gut microbiota and its effects on obesity, diabetes, heart disease, and cognitive function.

Our third area of emphasis is cartilage development and degeneration, with recent work focused on osteoarthritis, intervertebral disc degeneration, the transcriptional regulation of cartilage-specific genes, and the development of camelids as a model for spontaneous disc degeneration in humans.

 Memberships
  • International Society for Microbial Ecology, 2014-Present
  • American Society for Cell and Gene Therapy, 2013-Present
 Courses Taught

Fall 2014
  • MMBIO 494R: Mentored Research Section 003
Summer 2014
  • LFSCI 399R: Academic Internship Section 006
  • MMBIO 240: Molecular Biology Section 002
  • MMBIO 551R: Microbial Ecology Section 001
Winter 2014
  • BIO 662: Genomics, Mol Evol, Devel Bio Section 001
  • IAS 201R: Cultural Survey Section 034
  • MMBIO 390R: Readings in Molecular Biology Section 001
  • MMBIO 494R: Mentored Research Section 003
  • MMBIO 662: Genomics, Mol Evol, Devel Bio Section 001
  • PDBIO 662: Genomics, Mol Evol, Devel Bio Section 001
Fall 2013
  • BIO 661: Molecular Biology of the Cell Section 001
  • MMBIO 240: Molecular Biology Section 004
  • MMBIO 494R: Mentored Research Section 003
  • MMBIO 661: Molecular Biology of the Cell Section 001
  • PDBIO 661: Molecular Biology of the Cell Section 001

Selected Publications

Journal Articles

Stolworthy DK, Fullwood RA, Merrell TM, Bridgewater LC, Bowden AE. Biomechanical analysis of the camelid cervical intervertebral disc. Journal of Orthopaedic Translation. 2014 Dec;3(1):34-43. <website>

Bridgewater LC, Mayo JL, Evanson BG, Whitt ME, Dean SA, Yates JD, Holden DN, Schmidt AD, Fox CL, Dhunghel S, et al. A novel bone morphogenetic protein 2 mutant mouse, nBmp2NLStm, displays impaired intracellular Ca2+ handling in skeletal muscle. BioMed Research International. 2013 Nov;2013. <website>

Ricks ML, Farrell JT, Falk DJ, Holt DW, Rees M, Carr J, Williams T, Nichols BA, Bridgewater LC, Reynolds PR, et al. Osteoarthritis in Temporomandibular Joint of Col2a1 Mutant Mice. Archives of Oral Biology. 2013 Aug;58(9):1092-9.

Holt DW, Henderson ML, Stockdale CE, Ferrell JT, Kooyman DL, Bridgewater LC, Seegmiller RE. Osteoarthritis-like changes in the heterozygous sedc mouse associated with the HtrA1-Ddr2-Mmp-13 degradative pathway: a new model of osteoarthritis. Ostoeoarthritis and Cartilage. 2012 Jan;20:430-439. <website>

Holt DW, Henderson ML, Stockdale CE, Farrell JT, Kooyman DL, Bridgewater LC, Seegmiller RE. Osteoarthritis-like changes in the heterozygous sedc mouse associated with the HtrA1-Ddr2-Mmp-13 degradative pathway: A new model of osteoarthritis. Osteoarthritis and Cartilage. 2012 Jan;20:430-439. <website>

Felin JE, Mayo JL, Loos TJ, Jensen JD, Sperry DK, Gaufin SL, Meinhart CA, Moss JB, Bridgewater LC. Nuclear variants of bone morphogenetic proteins. BMC Cell Biology. 2010 Jan;11:20. <website>

Mayo JL, Holden DN, Barrow JR, Bridgewater LC. The transcription factor Lc-Maf participates in Col27a1 regulation during chondrocyte maturation. Experimental Cell Research. 2009 May;315:2293-2300. <website>

Identification of a novel RARE that requires an enhancer element mediator for transcriptional activation. Biochemical Journal. 2004;383:37-43.

Abstract

Olsen DS, Bailey KT, Nichols BA, Bridgewater LC. Lack of nuclear BMP-2 causes reduced spleen size. FASEB J. 2013 Apr;27:601.12.

Nichols BA, Goar WA, McCune BT, Bridgewater LC. Nuclear localized BMP2 promotes cell cycle progression. FASEB J. 2013 Apr;27:1029.1.

Kooyman DL, Ricks ML, Falk DJ, Farrell J, Rees MB, Carr JM, Williams DT, Bridgewater LC, Seegmiller RE. Premature osteoarthritis in the temporomandibular joint of heterozygous disproportionate micromelia mice. Experimental Biology. 2012 Apr;26:LB 115.

Conference Proceedings

Seegmiller RE, Niederhauser CM, Monta?o C, Bomsta BD, Wink AE, Sudweeks SN. Aggrecan expression in Dmm mice. supplementth ed. Nashville, Tennessee: Matrix Biology; 2006 Nov.

Presentations

Yates JD, Bridgewater LC. Evidence for a nuclear variant of decapentaplegic (dpp). Southwest Regional Meeting of the Society for Developmental Biology. Aurora, Colorado. 2014 .

Mayo JL, Nichols BA, Olsen DS, Cordner RD, Hancock CR, Weber KS, Wilson E, Edwards JG, Barrow JR, Bridgewater LC. The nBMP2 mutant mouse shows defects in intracellular calcium transport-regulated pathways. Southwest Regional Meeting of the Society for Developmental Biology. Aurora, Colorado. 2014 .

Bridgewater LC, Christensen L, Stolworthy DK, Fullwood RA, Merrell TM, Holland JG, Harmon LM, Robinson TF, Bowden AE. Development of an alpaca disc culture system for the study of intervertebral disc degeneration. Second International Spine Research Symposium. Philadelphia, PA. 2013 .

Fullwood RA, Stolworthy DK, Bowden AE, Bridgewater LC. Alpaca cervical spine anatomy: Shape, size, AF/NP ratio. Emerging Ideas in Biomedical Research Conference. Provo, Utah. 2013 .

Olsen DS, Bailey KT, Nichols BA, Bridgewater LC. Lack of nuclear BMP-2 causes reduced spleen size. American Society for Biochemistry and Molecular Biology. Boston, MA. 2013 .

Rogers AC, Nichols BA, Loos TJ, Bridgewater LC. Nuclear Bmp4 interacts with the SCF E3 ubiquitin ligase complex and inhibits the cell cycle. American Society for Biochemistry and Molecular Biology Annual Meeting. San Diego, California. 2012 .

Ricks M, Falk DJ, Farrell JT, Rees MB, Carr JM, Williams DT, Kooyman DL, Bridgewater LC, Seegmiller RE. Premature osteoarthritis in the temporomandibular joint of heterozygous disproportionate micromelia mice. American Society for Biochemistry and Molecular Biology Annual Meeting. San Diego, California. 2012 .