Julianne Grose

Research Interests

Cells have evolved complex mecha¬nisms that allow them to sense their metabolic status and regulate cellular responses appropri¬ately. A dysfunction in these regulatory process¬es has been shown to be involved in a variety of diseases including diabetes and cancer. Our lab is interested in the regulation of metabolism in response to the availability of nutrients and other factors affecting growth. We use model organ¬isms, such as Saccaromyces cerevisiae (baker’s yeast) and Salmonella typhimurium, to study key pathways involved in metabolic regulation. Pro¬teins and pathways are often conserved and thus our findings may aid in understanding metabolic regulation in other organisms, including humans.

Our lab focuses on two aspects of metabolic regulation. The first is the study of PAS kinase, a highly conserved sensory protein kinase that is involved in the regulation of glucose metabolism in both yeast and mammals. The PAS kinase protein has both a sensory and a regulatory domain. The sensory component consists of a PAS domain that may bind small molecule effecters. The PAS domain regulates an attached serine/threonine protein kinase that then modifies other proteins in order to regulate cellular processes in response to certain stimuli. Our goal is to further characterize the role PAS kinase plays in metabolic regulation by identifying specific mechanisms involved in its activation and function. The yeast, Saccaro-myces cerevisiae provides powerful genetic and biochemical tools for identifying PAS kinase related pathways and proteins.

The second aspect of metabolism we are studying is control of NAD and NADP levels within the cell. The vitamin niacin (B3) is a precursor to both NAD and NADP, which are essential for life in all organisms known. Together, these related cofactors serve in over 300 cellular reactions, several of which are central to basic metabolism. In addition to their roles as cofactors in metabolic reactions, NAD(P) also serve as allosteric regulators of many key metabolic reactions making control of NAD(P) levels critical to proper metabolic regulation. One of the key questions we are examining is why two very structurally related cofactor molecules have evolved (NAD and NADP differ by a single phosphate). It is thought these compounds arose in order to allow cells to differentially regulate metabolic process. In support of this hypothesis, NAD is primarily used for the production of cellular energy (ATP) while NADP is primarily used in reductive biosynthetic reactions that produce the molecu¬lar building blocks of the cell. Many of the pathways leading to the biosynthesis and recycling of NAD(P) have recently been described, however, several genes encoding key enzymes are still unknown. In addition, there are only laborious methods for accurately determining the levels of these compounds. We are interested in dis¬covering the genes encoding NAD(P)-related functions as well as in measuring the internal levels of these compounds in response to growth conditions and mutation.

Memberships

American Heart Association, 2012-Present
Science Education Alliance (SEA), 2010-Present

Honors and Awards

BYU Honors Program : Alcuin Fellowship
Brigham Young University : C. Joseph Rowberry Teaching and Learning Faculty Fellowship
Brigham Young University : Maesar Excellence in Teaching Award
NSF : First place award -NSF Community College Innovation Challenge (2017)
Faculty Womens Association : Faculty Womens Scholarship Award
BYU Technology Transfer Office : Technology Transfer Ward
IGEM : Gold medal
American Society for Microbiology : American Society for Microbiology Early-Career Faculty Travel Award
National Institute of Health : Ruth L. Kirschstein National Research Service Award Individual Fellowship
National Institute of Health : Multidisiplinary Cancer Training Fellow

Courses Taught

Winter 2019

HONRS 220: Unexpect Connect: Biol-Letters Section 002
MMBIO 195: Phage Comparative Genomics Section 001, 002
MMBIO 294R: Mentored Research Section 009
MMBIO 494R: Advanced Mentored Research Section 009
MMBIO 551R: Current Topics in MMBio Section 004, 006
MMBIO 695R: Research Section 009
MMBIO 699R: Master's Thesis Section 009
MMBIO 799R: Dissertation Section 009

Fall 2018

HONRS 220: Unexpect Connect: Biol-Letters Section 002
MMBIO 194: Phage Discovery Section 001, 002
MMBIO 494R: Advanced Mentored Research Section 009
MMBIO 695R: Research Section 009
MMBIO 699R: Master's Thesis Section 009
MMBIO 799R: Dissertation Section 009

Summer 2018

MMBIO 494R: Mentored Research Section 009

Spring 2018

MMBIO 494R: Mentored Research Section 009

Publications

Journal Articles

Pape JA, Newey CR, Burrell HR, Workman A, Perry K, Bikman BT, Bridgewater LC, Grose JH. 2018. Per-Arnt-Sim Kinase (PASK) Deficiency Increases Cellular Respiration on a Standard Diet and Decreases Liver Triglyceride Accumulation on a Western High-Fat High-Sugar Diet. Nutrients. 10(12).

DeMille D, Pape JA, Bikman BT, Ghassemian M, Grose JH. 2018. The Regulation of Cbf1 by PAS Kinase Is a Pivotal Control Point for Lipogenesis Versus Respiration in Saccharomyces cerevisiae. doi:10.1534/g3.118.200663

Berg JA, Merrill BD, Breakwell DP, Hope S, Grose JH. 2018. A PCR-Based Method for Distinguishing between Two Common Beehive Bacteria, Paenibacillus larvae and Brevibacillus laterosporus. 84(22). doi:10.1128/AEM.01886-18

Sharma R, Berg JA, Beatty NJ, Choi MC, Cowger AE, Duncan SG, Fajardo CP, Ferguson HP, Galbraith T, Herring JA, et al. 2018. Genome Sequences of Nine Erwinia amylovora Bacteriophages. 7(14). doi:10.1128/MRA.00944-18

Merrill BD, Fajardo CP, Hilton JA, Payne AM, Ward AT, Walker JK, Dhalai A, Imahara C, Mangohig J, Monk J, et al. 2018. Complete Genome Sequences of 18 Paenibacillus larvae Phages from the Western United States. 7(13). doi:10.1128/MRA.00966-18

Yost DG, Chang C, LeBlanc L, Cassin E, Peterman C, Rai P, Salisbury A, Barroga N, Cisneros R, Fersini J, et al. 2018. Complete Genome Sequences of Paenibacillus larvae Phages Halcyone, Heath, Scottie, and Unity from Las Vegas, Nevada. 7(12). doi:10.1128/MRA.00977-18

Stamereilers C, Fajardo CP, Walker JK, Mendez KN, Castro-Nallar E, Grose JH, Grose JH, Hope S, Tsourkas PK. 2018. Genomic Analysis of 48 Paenibacillus larvae Bacteriophages. Viruses. 10(7). doi:10.3390/v10070377

Arens DK, Brady TS, Carter JL, Pape JA, Robinson DM, Russell KA, Staley LA, Stettler JM, Tateoka OB, Townsend MH, et al. 2018. Characterization of two related Erwinia myoviruses that are distant relatives of the PhiKZ-like Jumbo phages. 13(7):e0200202. doi:10.1371/journal.pone.0200202

Willis SD, Stieg DC, Ong KL, Shah R, Strich AK, Grose JH, Cooper KF. 2018. Snf1 cooperates with the CWI MAPK pathway to mediate the degradation of Med13 following oxidative stress. 5(8):357-370. doi:10.15698/mic2018.08.641

Walker JK, Merrill BD, Berg JA, Dhalai A, Dingman DW, Fajardo CP, Graves K, Hill HL, Hilton JA, Imahara C, et al. 2018. Complete Genome Sequences of Paenibacillus larvae Phages BN12, Dragolir, Kiel007, Leyra, Likha, Pagassa, PBL1c, and Tadhana. 6(24). doi:10.1128/genomeA.01602-17

Stieg DC, Willis SD, Ganesan V, Ong KL, Scuorzo J, Song M, Grose JH, Strich R, Cooper KF. 2018. A complex molecular switch directs stress-induced cyclin C nuclear release through SCF^Grr1-mediated degradation of Med13. 29(3):363-375. doi:10.1091/mbc.E17-08-0493

Dedrick RM, Jacobs-Sera D, Bustamante CA, Garlena RA, Mavrich TN, Pope WH, Reyes JC, Russell DA, Adair T, Alvey R, et al. 2017. Prophage-mediated defence against viral attack and viral counter-defence. Nature Microbiology. 2:16251. doi:10.1038/nmicrobiol.2016.251

Grose JH, Casjens S. 2016. Contributions of P2- and P22-like prophages to understanding the enormous diversity and abundance of tailed bacteriophages. Virology. 496:255-7.

Merrill BD, Ward AN, Grose JH, Hope S. 2016. Software-based analysis of bacteriophage genomes, physical ends, and packaging strategies. BMC Genomics. 17:679. doi:0.1186/s12864-016-3018-2

Berg JA, Merrill BD, Crockett JT JT, Esplin KP, Evans MR, Heaton KE, Hilton JA, Hyde JR, McBride MS, Schouten JT, et al. 2016. Characterization of Five Novel Brevibacillus Bacteriophages and Genomic Comparison of Brevibacillus Phages. PLoS One. 1(6):e015683. doi:0.1371/journal.pone.0156838

Grose JH, Langston K, Wang X, Squires S, Mustafi S, Hayes W, Neubert J, Fischer S, Fasano M, Saunders G, et al. 2015. Characterization of the Cardiac Overexpression of HSPB2 Reveals Mitochondrial and Myogenic Roles Supported by a Cardiac HspB2 Interactome. 10(10). doi:10.1371/

Grose JH, Merrill BD, Berg JA, Graves KA, Ward AT, Hilton JA, Wake BN, Grose JH, Breakwell DP, Burnett SH. 2015. Genome Sequences of Five Additional Brevibacillus laterosporus Bacteriophages. 22(3(5)):e01146-15. doi:10.1128/genomeA.01146-15

Pope WH, Bowman CA, Russell DA, Jacobs-Sera D, Asai DJ, Cresawn SG, Jacobs WR, Hendrix RW, Lawrence JG, Hatfull GF, et al. 2015. Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity. Elife.

Merrill BD, Berg JA, Kiel A Graves AW, Hilton JA, Wake BN, Grose JH, Grose JH, Breakwell DP, Hope S. 2015. Genome Sequences of Five Additional Brevibacillus laterosporus Bacteriophages. Genome Announcement. :15. doi:10.1128/genomeA.01146-15

DeMille D, Badal BD, Evans JB, Mathis AD, Anderson JF, Grose JH. 2015. PAS kinase is activated by direct Snf1-dependent phosphorylation and mediates inhibition of TORC1 through the phosphorylation and activation of Pbp1. doi:10.1091/mbc.E14-06-1088

Grose JH, Belnap DM, Jensen JD, Mathis AD, Prince JT, Merrill BD, Hope S, Breakwell DP. 2014. The Genomes, Proteomes, and Structures of Three Novel Phages That Infect the Bacillus cereus Group and Carry Putative Virulence Factors. Journal of Virology. 88:11846-11860.

Grose JH, Jensen GS, Hope S, Breakwell DP. 2014. Genomic comparison of 93 Bacillus phages reveals 12 clusters, 14 singletons and remarkable diversity. 15:855. doi:10.1186/1471-2164-15-855

Grose JH, Casjens SR. 2014. Understanding the enormous diversity of bacteriophages: The tailed phages that infect the bacterial family Enterobacteriaceae. 468-470C:421-443. doi:10.1016/j.virol.2014.08.024

Merrill BD, Grose JH, Breakwell DP, Hope S. 2014. Characterization of Paenibacillus larvae bacteriophages and their genomic relationships to firmicute bacteriophages. BMC Genomics. 15(Aug 30):745.

DeMille D, Bikman BT, Mathis AD, Prince JT, Mackay JT, Sowa SW, Hall TD, Grose JH. 2014. A comprehensive protein-protein interactome for yeast PAS kinase 1 reveals direct inhibition of respiration through the phosphorylation of Cbf1. Molecular Biology of the Cell. 25(14):2199-2215.

Grose JH, Jensen JD, Merrill BD, Fisher J, Hope S, Breakwell DP. 2014. The Genomes of Three Novel Bacillus cereus Bacteriophages. Genome Announcements. 2(1):2. doi:10.1128/genomeA.01118-13

DeMille D, Grose JH. 2013. PAS kinase: a nutrient sensing regulator of glucose homeostasis. IUBMB Life. 65(11):921-9. doi:10.1002/iub.1219

Breakwell DP, Barrus EZ, Benedict AB, Brighton AK, Fisher JN, Gardner AV, Kartchner BJ, Ladle KC, Lunt BL, Merrill BD, et al. 2013. Genome Sequences of Five Cluster B1 Mycobacteriophages. Genome Announcements. 1(6). doi:10.1128/genomeA.00968-13

Sheflo MA, Gardner AV, Merrill BD, Fisher JN, Lunt BL, Breakwell DP, Grose JH, Hope S. 2013. Complete Genome Sequences of Five Paenibacillus larvae Bacteriophages. Genome Announcements. 1(6). doi:10.1128/genomeA.00668-13.

Banerjee MS, Grose JH, Zhang H, Pratt GW, Sadoshima J, Christians E, Benjamin I. 2013. Aggregate-prone R120GCRYAB triggers multifaceted modifications of the Thioredoxin System. Antioxid Redox Signal.

Smith KC, Castro-Nallar E, Fisher JN, Breakwell DP, Grose JH, Hope S. 2013. Phage cluster relationships identified through single gene analysis. BMC Genomics. 14(410):15.

Hatfull G, Grose JH, Hope S, Breakwell DP. 2012. Complete genome sequences of 138 mycobacteriophages. Journal of Virology. 86(4):2382-2384.

Grose JH, Rutter J. 2010. The Role of PAS Kinase in PASsing the Glucose Signal. Sensors. 10(6):5668-5682.

Book Chapter

Grose JH. 2010. Regulation of NAD(P) Metabolism in Salmonella enterica. S. Maloy, K. Hughes and J. Casadesus, editors. Regulation of NAD(P) Metabolism in Salmonella enterica. In The Allure of Bacterial Genetics. Washington D.C.: ASM Press. p. 22 pages

Conference Proceedings

Adebayo J, Southwick T, Chetty V, Yeung E, Yuan Y, Goncalves J, Grose JH, Prince JT, Stan G, Warnick SC. 2012. Dynamical Structure Function Identifiability Conditions Enabling Signal Structure Reconstruction.

Journal Contribution, Nonfiction

Esplin IN, Berg JA, Sharma R, Allen RC, Arens DK, Ashcroft CR, Bairett J, Bickmore M, Bloomfield TJ, Brady TS, et al. 2017. Genome Sequences of 19 Novel Erwinia amylovora Bacteriophages. Genome Announc. 5(46). doi:10.1128/genomeA.00931-17

Other

Earley BJ, Engle JM, Smith KC, Lunt BL, Fisher JN, Payne II DE, Breakwell DP, Hope S, Grose JH. 2011. Mycobacterium phage Jebeks, complete genome. JN572061st ed.

Grose JH, Fried-Petersen H, Adair TL, Anders KR, Aley SB, Bratsch SA, Clase KL, Coleman JM, Debro LH, Dellis S, et al. 2011. Mycobacterium phage Wee, complete genome. NC_014901.1st ed.

Presentations

Fajardo C, Meredith S, Roll C, Griffitts JS, Hope S, Grose JH, Breakwell DP. Proof of Concept: Determining Phage Adsorption Using Flow Cytometry. Intermountain ASM TriBranch Meeting. Durango, CO. 2018 .

Franson J, White J, Ong KL, Choksi N, Hilton A, Rees A, Resolme J, Zhao J, Sevey R, Olsen K, et al. Effect of Diet, Genes, and Microbiota on Glucose Tolerance in a Mouse Model with a Genetically Increased Metabolic Rate. LDS Life Science Research Symposium. Lehi, Utah. 2016 .

Franson J, Sevey R, Zhao J, White J, Hilton A, Rees A, Ong KL, Choksi N, Grose JH, Bridgewater LC. Effect of Diet on the Microbiota of a Mouse Model with a Genetically Increased Metabolism Rate. American Society of Microbiology Intermountain Branch Meeting. Salt Lake City, Utah. 2016 .

Zhao J, Grossarth S, Grose JH, Bridgewater LC. Phage hunting through the human gut. Phage Phield Day. Provo, Utah. 2016 .

White J, Franson J, Rees A, Hilton A, Ong KL, Choksi N, Resolme J, Zhao J, Grose JH, Bridgewater LC. PAS kinase and its role in the development of diabetes and obesity in mice. Utah Conference for Undergraduate Research. Salt Lake City, Utah. 2016 .

Grose JH, Bikman BT, Demille D, Pattison J. The role of PAS kinase in controlling cellular respiration. Analytical Genetics. Rotorua, New Zealand. 2016 .

Grose JH, Bikman BT, DeMille D. The role of PAS kinase in controlling cellular respiration. Multifaceted Mitochondria. Chicago, Illinois. 2015 .

Grose JH, Pattinson J, DeMille D, Bikman BT. The transcription factor centromere binding factor 1 (Cbf1) as a central point of control to upregulate mitochondrial activity and decrease lipid biogenesis in the yeast Saccharomyces cerevisiae”. Tri-Branch ASM Meeting. Durgano, Colorado. 2015 .

Chetty V, Adebayo J, Mathis A, Demille D, Morley S, Anthonymuthu T, Yuan Y, Goncalves J, Grose JH, Prince JT, et al. In-Silico Robust Reconstruction of the Per-Arnt-Sim Kinase Pathway using Dynamical Structure Functions. Foundations of Systems Biology and Engineering. Tsuruoka, Japan. 2012 .

Grose JH, Kooyman DL, Anderson J, Buckley A, Pezoa Cabeza Y, Emery H, Fullwood R, Hecht K, Jackson K, Jones E, et al. E. colin: A two circuit system for colin cancer detection. IGEM Regional Jamboree. Standford University. 2012 .

Mathis A, Anthonymuthu TS, Morley S, Southwick T, DeMille D, Grose JH, Adebayo J, Warnick SC, Prince JT. Definitive Network Reconstruction of the Yeast PAS Kinase Network via Mass Spectrometry Proteomics and Phosphoproteomics. US HUPO: The Future of Proteomics. San Francisco, California. 2012 .

Grose JH, Kooyman DL, Biggs M, Chamberlain C, Merrill M, Sabin D, Sabin M, Roberts J, Williams L, Alley A, et al. E. colinoscopy. Current topics in Chemistry. BYU. 2011 .

Grose JH, Kooyman DL, Biggs M, Chamberlain C, Merrill M, Sabin D, Sabin M, Roberts J, Williams L, Alley A, et al. Evolving a thermoswitch sensitive to narrow temperature shifts. IBE Western Regional Student Conference. Utah State University. 2011 .

Grose JH, Kooyman DL, Biggs M, Chamberlain C, Merrill M, Sabin D, Sabin M, Roberts J, Williams L, Alley A, et al. E. colinoscopy. IGEM Worldchampionship Jamboree. MIT. 2011 .

Grose JH. The role of yeast PAS kinase in PASsing glucose. Analytical Genetics International Conference. Carmona, Spain. 2011 .

Grose JH, Kooyman DL, Biggs M, Chamberlain C, Merrill M, Sabin D, Sabin M, Roberts J, Williams L, Alley A, et al. E. colinoscopy. IGEM Regional Jamboree. Indianapolis. 2011 .

Brighton AK, Fisher J, Lunt BL, Taylor MA, Smith KC, Breeanna Baker EB, Chapman KM, Drake EA, Jackson KR, Kartchner BJ, et al. Additional Evidence for Frameshifts in A2 and Gene Mosaicism in F Mycobacteriophage. Science Education Alliance (SEA) Annual Symposia. Janelia Farm, Ashburn, VA. 2011 .

Grose JH, Breakwell DP, Hope S. Out of the SEA: Getting Students to Crawl on Land. Science Education Alliance (SEA) Annual Symposia. Janelia Farm, Ashburn, VA. 2011 .

Kartchner BJ, Kiser JT, Kiser CD, Wade McDaniel S, Taylor MA, Fisher J, Lunt B, Hope S, Grose JH, Breakwell DP. Clustering of Mycobacteriophage in the Utah Landscape. ASM Intermountain Branch Meeting. Weber State University. 2011 .

Smith KC, Hope S, Grose JH, Breakwell DP. Degenerate PCR Primers to Identify Mycobacteriophage Clusters and Sub-Clusters. ASM Intermountain Branch Meeting. Weber State University. 2011 .

Brighton AK, Vance KS, Merridee Parker KJ, Steck RP, Ormsby WR, Taylor MA, Fisher J, Lunt B, Hope S, Grose JH, et al. Gene Mosaicism Demonstrated in Mycobacteriophage Shauna1. ASM Intermountain Branch Meeting. Weber State University. 2011 .

Kitchen JC, Brighton AK, Chapman KM, Baker B, Taylor MA, Fisher J, Lunt B, Hope S, Grose JH, Breakwell DP. Morphological Traits of Mycobacteriophage Clusters and Sub-Clusters. ASM Intermountain Branch Meeting. Weber State University. 2011 .

Zane Barrus E, Sheide MG, Taylor MA, Fisher J, Lunt B, Hope S, Grose JH, Breakwell DP. Shauna1 Mycobacteriaphage holin gene confirms common ancestry of all F cluster phage. ASM Intermountain Branch Meeting. Weber State University. 2011 .

Chapman KM, Baker B, Drake EA, Kitchen J, Taylor MA, Fisher J, Lunt B, Hope S, Grose JH, Breakwell DP. TA17A: A Unique Member of the Mycobacteriophage Sub-Cluster A2. ASM Intermountain Branch Meeting. Weber State University. 2011 .